Microcarrier culture of fish cells and viruses in cell culture bioreactor. |
| |
Authors: | Z Chen Y Chen Y Shi X Yie G Yang |
| |
Affiliation: | Research Institute of Biochemical Engineering, East China University of Chemical Technology, Shanghai. |
| |
Abstract: | This article deals with the culture of grass carp (Ctenopharyngodon idellus) lip and embryo cells on Cytodex 3 and GT-2 microcarriers in a 1.5-L cell culture bioreactor to propagate grass carp hemorrhage virus. The cells and viruses were successfully cultivated at 26 degrees C, pH 7.0, and dissolved oxygen 40% of air saturation. The cell density achieved was as high as 7.4 x 10(6) cells/mL, and the virus titre reached 6.75 log LD50/0.5 mL from an initial 3.00 log LD50/0.5 mL. The results present broad prospects for fish virus vaccine production. |
| |
Keywords: | |
|
|