Computer-assisted simulations of phosphofructokinase-1 kinetics using simplified velocity equations |
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| Affiliation: | 1. Water Research Institute, C.N.R., Via Salaria km 29.300, CP 10, 00015, Monterotondo Stazione, Rome, Italy;2. Department of Chemical Engineering, Queen''s University, Kingston, Ontario K7L 3N6, Canada;1. Liverpool John Moores University, Liverpool, United Kingdom;2. Edge Hill University, Ormskirtk, United Kingdom |
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| Abstract: | Equations useful for simulating the kinetic behavior of phosphofructokinase are presented. The equations, which are based on the concerted transition (symmetry) model for allosteric enzymes, account for substrate inhibition by MgATP, cooperative binding by F-6-P, activation by F-2,6-P2, and deinhibition by AMP. Velocity calculations can be performed using either a spreadsheet program (e.g., MS Excel) or a web-based program (e.g., Authorware). Both approaches are illustrated. |
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