Stimulating action of methyl 12, 12, 12-trifluorofarnesoate on in vitro juvenile hormone III biosynthesis in blattella germanica |
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Authors: | Xavier Bell s,Francisco Camps,Josefina Casas,Bernard Mauchamp,Maria-Dolors Piulachs,Angel Messeguer |
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Affiliation: | Xavier Bellés,Francisco Camps,Josefina Casas,Bernard Mauchamp,Maria-Dolors Piulachs,Angel Messeguer |
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Abstract: | Methyl 12, 12, 12-trifluorofarnesoate (MTFF) at a dose of 10 μM, stimulated in vitro juvenile hormone (JH) release in corpora allata (CA) from 6-day-old, freshly ecdysed, and 8-day-old (period of ootheca transport) adult virgin females of Blattella germanica. In addition, MTFF also induced intraglandular accumulation of JH and MF in treated CA. Trifluorofarnesoic acid (TFFA) and trifluorofarnesol (TFF) exhibited the same properties, although to a lesser extent than MTFF. The detection of MTFF in TFFA-treated CA suggested that TFFA and TFF were biotransformed into MTFF by the CA enzymatic system and that this ester might be responsible for the activity observed. Equivalent experiments carried out with farnesoic acid (FA) resulted in a more significant stimulation of JH production. This is not surprising, because exogenous FA is readily epoxidized at C10-C11 double bond and methylated to afford JH. Conversely, analytical data have shown that the C6-C7 double bond of MTFF is epoxidized by the CA enzymatic system, whereas that at C10-C11 remains practically unaltered. |
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Keywords: | German cockroach corpora allata farnesoic acid 12, 12, 12-trifluorofarnesoic acid 12, 12, 12-trifluorofarnesol |
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