Abstract: | This study compares urinary E1-3G and serum E2 measurements in monitoring ovulation induction in an in vitro fertilization programme (FIVET). We used an RIA kit, ESTR.CTK2 (Sorin, Italy), for the daily determination of serum E2, and an LA kit, Fertilux (Bouty, Milan), for the determination of E1-3G in early morning urine samples using a timed and measured volume urine collection procedure. A significant correlation was found between serum E2 as measured by RIA and urinary E1-3G as measured by LIA. Considering the RIA disadvantages (the short half-life of the reagents, the hazards of handling radioactive materials, the inconvenience of frequent venepuncture for the patients) the LIA measurement seems to be a useful method in a FIVET programme. |