Lys-tRNA4 levels and cell division in mouse 3T3 cells |
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Authors: | Christine Conlon-Hollingshead BJ Ortwerth |
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Institution: | Departments of Ophthalmology and Biochemistry, University of Missouri, Columbia, MO 65201, USA |
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Abstract: | tRNA4lys is an isoaccepting tRNAlys which has been proposed as a necessary requirement for cell division in mammalian cells. We have measured the levels of this tRNAlys during the growth cycle of mouse 3T3 fibroblasts. High levels of tRNA4lys were seen throughout exponential growth. However, a marked decrease in tRNA4lys occurred 24 h before the cells became confluent. This decrease was observed in three different 3T3 cell lines, but was not seen in a transformed 3T3 cell line. Trypsinization and replating of contact-inhibited cells returned tRNA4lys to the levels characteristic of exponential cells. Data from these and other cell lines show a direct relationship between the levels of tRNA4lys and the growth rate of cells in culture. |
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