Schistosoma mansoni: Characterization of two circulating polysaccharide antigens and the immunological response to these antigens in mouse,hamster, and human infections |
| |
Authors: | A.M. Deelder D. Kornelis E.A.E. Van Marck P.C. Eveleigh J.G. Van Egmond |
| |
Affiliation: | Medical Center, Department of Parasitology, Leiden University, Rapenburg 33, Leiden, The Netherlands;Department of Histopathology and Experimental Schistosomiasis, Prins Leopold Institute of Tropical Medicine, Antwerp, Belgium;Medical Center, Department of Parasitology, Leiden University, Rapenburg 33, Leiden, The Netherlands |
| |
Abstract: | In this study the nature and occurrence of two circulating polysaccharide antigens of Schistosoma mansoni, circulating anodic antigen (CAA) and circulating cathodic antigen (CCA), and the immunological response to these antigens in mouse, hamster, and human infections were investigated. Both CAA and CCA showed a large molecular weight range, less than 50,000 to over 300,000 for CAA and 50,000 to over 300,000 for CCA, possibly representing monomers and polymers. CAA and CCA could be purified from the trichloroacetic acid-soluble fraction of adult worm antigen (AWA-TCA) by means of DEAE ion exchange chromatography. The presence of at least two other components in AWA-TCA was shown. Both CAA and CCA were found to be gut associated, and could be demonstrated in the vomitus and in the excretory and secretory antigens of adult worms. Both antigens were present in the kidney eluates of infected hamsters, while CCA could normally be detected in the urine of these hamsters and CAA only occasionally. CAA was demonstrated in the Kupffer cells of the livers of infected mice and hamsters. Antibodies against CAA and CCA were shown in mouse, hamster, and human infections. In human infections specific IgM titers against these antigens were especially elevated in children and in recent infections of adults. |
| |
Keywords: | Trematode Blood fluke Gel filtration Ion exchange chromatography Immunoelectrophoresis Immunofluorescence DASS system ELISA IHA Polysaccharide antigens Antibodies Immune complexes Hamster Mouse Human Circulating anodic antigen CAA Circulating cathodic antigen CCA TCA-soluble fraction of AWA V worm vomitus TAA tegument-associated antigens (KGl extraction) ESA excretory and secretory antigens of adult worms KE ash hamster antiserum asm mouse antiserum ass sheep antiserum |
本文献已被 ScienceDirect 等数据库收录! |
|