The basis of silver staining of bacterial lipopolysaccharides in polyacrylamide gels |
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| Authors: | Dr. Andrew M. Kropinski Denise Berry E. Peter Greenberg |
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| Affiliation: | (1) Department of Microbiology and Immunology, Queen's University, K7L 3N6 Kingston, Ontario, Canada;(2) Carbohydrate Research Institute, Queen's University, K7L 3N6 Kingston, Ontario, Canada;(3) Department of Microbiology, New York State College of Agriculture and Life Sciences, Cornell University, Ithaca, New York, USA |
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| Abstract: | When cast in a polyacrylamide gel, whole lipopolysaccharide (LPS) and the lipid A fraction of LPS fromSalmonella typhimurium andEscherichia coli O111 B4 reacted with the silver stain described by Tsai and Frasch [11]. However, the polysaccharide fractions released from the LPS by acid hydrolysis were not stained. This is inconsistent with the previously believed notion that the polysaccharide component is that which reacts with the silver stain. |
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