pH influences growth and plasmid stability of recombinant Lactococcus lactis subsp. lactis |
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| Authors: | Catherine Béal Christine D'Angio Georges Corrieu |
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| Institution: | (1) Laboratoire de Génie et Microbiologie des Procédés Alimentaires, INA P-G, INRA, Thiverval-Grignon, France |
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| Abstract: | Continuous cultures of a recombinant Lactococcus lactis subsp. lactis strain were performed to display the effect of the fermentation pH on the specific growth rate and plasmid stability. The proportion of plasmid pIL252 bearing cells decreased exponentially with the number of generations. The influence of the pH on the rate of loss of plasmid pIL252 and on the specific growth rate of L. lactis IL2682 was described by second order polynomial equations. Optimal pH for the growth and plasmid stability were 6.39 and 6.41 respectively. © Rapid Science Ltd. 1998 |
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