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Some characteristics of a raw starch digestion inhibitory factor fromAspergillus niger
Authors:Sirintornthep Towprayoon  Badal Chandra Saha  Yusaku Fujio  Seinosuke Ueda
Affiliation:(1) Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, 812 Fukuoka, Japan;(2) Present address: Department of Biology, Faculty of Science, Khon Kaen University, 40002 Khon Kean, Thailand;(3) Present address: Michigan Biotechnology Institute, 48909 Lansing, Michigan, USA;(4) Department of Applied Microbial Technology, Faculty of Engineering, Kumamoto Institute of Technology, 860 Kumamoto, Japan
Abstract:Summary The effect of an inhibitory factor (IF) fromAspergillus niger 19 on raw starch digestion by pure glucoamylase I of blackAspergillus, pure glucoamylae ofRhizopus niveus, bacterial agr-amylase, fungal agr-amylase and various combination was investigated. The IF caused higher inhibition of raw starch hydrolysis by the combined action of glucoamylase and fungal agr-amylase than of hydrolysis by the individual enzymes. A protein moiety of IF might play an active part in this inhibition phenomenon. The IF was bound to starch granules, preventing hydrolysis by the enzymes, and caused decreased raw starch hydrolysis yields.
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