Somatic embryogenesis in Araucaria angustifolia |
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| Authors: | A. L. Wendt dos Santos N. Steiner M. P. Guerra K. Zoglauer B. M. Moerschbacher |
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| Affiliation: | (1) Institute of Plant Biochemistry and Biotechnology, University of Muenster, D-48149 Muenster, Germany;(2) Department of Plant Science, Federal University of Santa Catarina, 88034-001 Florianópolis, Brazil;(3) Institute of Biology, Humboldt University, D-10115 Berlin, Germany |
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| Abstract: | Immature and mature zygotic embryos were used as source of explants for induction of somatic embryogenesis in Araucaria angustifolia. Embryogenic cultures (EC) were only obtained from immature zygotic embryos. Basic medium, carbon source, and genotype showed a significant influence on the formation of stage I somatic embryos (SE). When EC were submitted to maturation conditions, SE continued their individual development until stage II, but mature embryos were not obtained. Proteins secreted by embryogenic cultures were, to a certain degree, genotype specific and included an extracellular class IV chitinase and β-1-3-glucanase. |
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| Keywords: | Araucariaceae cell suspension cultures extracellular proteins |
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