水稻细胞质雄性不育系及其保持系幼穗发育过程中内源激素的变化

利用酶联免疫测定技术研究了水稻细胞质雄性不育系珍汕97A及其保持系珍汕97B在幼穗发育过程中叶片、幼穗和花药中内源IAA、GA1＋4、ABA和iPAs含量的动态变化。结果表明，1）从幼穗发育的雌雄蕊形成期到三核花粉期，保持系叶片中IAA水平高于不育系，并在二核花粉期最高；在幼穗和花药中也以保持系为高。2）保持系与不育系叶片中GA1＋4含量变化趋势相似，均为先升后降，但从单核到三核花粉期以保持系为高；在幼穗和花药中也都以保持系为高。3）不育系叶片中ABA水平在幼穗发育早期明显高于保持系，中期与保持系相近，后期又高于保持系；在幼穗和花药中也都以不育系为高。4）保持系叶片、幼穗和花药中iPAs含量始终显著高于不育系。5）保持系叶片中IAA＋GA1＋4＋iPAs与ABA之比值也始终高于不育系。提示不育系叶片、幼穗和花药中IAA、GA1＋4、iPAs和ABA含量出现了异常，且IAA、GA1＋4和iPAs亏缺以及ABA盈积可能与水稻细胞质雄性不育发生有关。

CHANGES IN PHYTOHORMONES IN CYTOPLASMIC MALE STERILE RICE AND ITS MAINTAINER LINE DURING PANICLE DEVELOPMENT

Contents of IAA, GA1 4, ABA and iPAs were measured by enzyme-linked irnmunosorbent assay (ELISA) in leaves, panicles and anthers of cytoplasndc male sterile (CMS) rice Zhenshan 97A and its maintainer Zhenshan 97B during panicle development.From stamen and pistil formation to trinucleate pollen stages, IAA content in leaves of CMS line was shown to be lower than that of maintainer line, and the highest level was observed at binucleate pollen stage in maintainer line. So were IAA contents in anthers and panicles. Changes in GAsimilar pattern, i.e. in both lines, the level of GA1 4 rose at first, then dropped, however,the highest levels were found in leaves as well as in panicles and anthers of maintainer line during uninucleate to trinucleate stages. ABA level in leaves of CMS line were obviously higher than that in maintainer one at early panicle developmental stage, and was at nearly the same level in both lines at griddle stage, then again still higher than that in maintainer line. The levels of ABA in panicles and anthers in CMS line were also higher than those in maintainer line. As to the conteflts of iPAs. in leaves, panicles and anthers, markedly lower level in CMS line than in maintainer line was found at all stages. The ratios of IAA GA1 4 iPAs to ABA in leaves, panicles and anthers of CMS line were also lower than those in maintainer one. It is proposed that the changes in contents of these phytohormones in CMS line are abnormal, and IAA, GA1 4 and iPAs deficiency and ABA accumulation aught be related to the generation of CMS in rice.