Characterization of soybean tissue culture cell lines resistant to methionine analogs |
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Authors: | James T Madison John F Thompson |
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Institution: | (1) Agricultural Research Service, United States Department of Agriculture, U.S. Plant, Soil & Nutrition Laboratory, Tower Road, 14853 Ithaca, NY, USA |
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Abstract: | Several hundred soybean Glycine max (L.) Merr.] cell lines resistant to ethionine were isolated either with or without chemical mutagenesis. of these, 26 were found to contain 2 to 22 times higher than normal levels of uncombined methionine. These 26 cell lines also contained higher than normal levels of S-adenosylmethionine and S-methylmethionine, but the levels of free lysine, threonine, cysteine, valine, tyrosine and phenylalanine were not elevated. Isoleucine levels were only slightly elevated. These results suggest that the regulation of methionine synthesis in vivo is more likely to be later in the pathway (after homoserine phosphate) than early in the pathway.Abbreviations AdoMet
S-adenosylmethionine
- SMM
S-methylmethionine
- trp
tryptophan
- met
methionine
- thr
threonine
- val
valine
- phe
phenylalanine
- lys
lysine
- ile
isoleucine
- cys
cysteine |
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