Polymorphisms in grapevine DNA detected by the RAPD PCR technique |
| |
| Authors: | Graham G Collins Robert H Symons |
| |
| Institution: | (1) Department of Horticulture, Viticulture and Oenology, University of Adelaide, 5064 Glen Osmond, SA, Australia;(2) Department of Plant Science, University of Adelaide, 5064 Glen Osmond, SA, Australia;(3) Cooperative Research for Viticulture, Waite Campus, University of Adelaide, 5064 Glen Osmond, SA, Australia |
| |
| Abstract: | A sensitivie, reproducible technique is described for detecting polymorphisms between the nuclear DNA of grapevine isolates
using the RAPD PCR technique. Unique fingerprints of a number of cultivars were readily distinguished by using either single
primers or mixtures of two primers. The method will be used to provide a databank of fingerprints for the rapid identification
of grapevine cultivars, and to develop phylogenetic relationships. It will also be extended and modified in an attempt to
detect polymorphisms between DNAs of clonal selections of individual cultivars. |
| |
| Keywords: | DNA grapevine PCR polymorphisms RAPD Vitis |
| 本文献已被 SpringerLink 等数据库收录！ |
|
