Kinetic aspects of inhibition of the phytopathogenic fungi growth by rhizosphere bacteria |
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Authors: | O. M. Minaeva E. L. Akimova E. V. Evdokimov |
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Affiliation: | (1) Tomsk State University Research Institute of Biology and Biophysics, 634050 Tomsk, Russia |
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Abstract: | Kinetics of growth inhibition of fungi Fusarium and Bipolaris caused by bacteria Pseudomonas sp. V-6798 and Azotobacter chroococum V-2272 D on dense nutrient media, both in single-crop system and by coinoculation, was demonstrated. The speed of fungal colonies growth as a function of bacteria concentration in inoculate was shown to be in accordance with the Ierysalimskii modified equation. The degree of antagonistic activity was suggested to be assessed by the constant of inhibition (K i ) and residual rate of fungi growth. Constant of inhibition of fungal growth by bacteria varied within 10–100 cells/ml for observed species. More effective fungistatic influence of bacterial strains in combined culture was observed. Parameters reported in the present study allow comparing the degree of bacteria antifungal activity in vitro. Suggested screening method could be used for selection of bacteria as activity biofungicide and while selecting biomedication for defined plant pathogen disruption. |
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