Tumorigenesis of rat mammary epithelial cells by N-nitroso-N-methylurea in anin vitro system: Characterization of the microtumors |
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Authors: | Jeffrey R Laduca Dilip K Sinha |
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Institution: | (1) Department of Breast Surgery and Breast Cancer Research Unit, Roswell Park Cancer Institute, Elm and Carlton Streets, 14263 New York, Buffalo |
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Abstract: | Summary Chemical carcinogenesis is a lengthy process that involves the rather loosely defined stages of initiation, promotion, and
progression. Several model systems of mammary carcinogenesis have been designed to elucidate the mechanisms of chemical carcinogenesis.
Most of these systems have included animal models. While organ specific chemical carcinogenesis can be initiated in these
systems, the subsequent stages of promotion and progression are difficult to study in detail. Investigations onin vitro carcinogenesis have shown transformation of mammalian cells in culture; the transformational event, however, is difficult
to discern within the monolayer culture. We have recently reported the development of anin vitro carcinogenesis system that allows both the initiation as well as the progression of mammary cells in a collagen gel matrix
culture system. The cells transformed by a chemical carcinogen develop into discernible microtumors with the three dimensions
of a collagen gel culture. Isolation of these microtumors from the collagen gel an subsequent culture in monolayer has produced
cells capable of colony formation in soft agar. The present study further characterizes these microtumors originatedin vitro by analysis of cell growth kinetics versus parallel control cells. In addition, flow cytometric and cytogenetic studies have
been performed to investigate the chromosomal stability of these cells. It was also observed that the microtumors, producedin vitro from mammary epithelial cells of an inbred strain of rats, show the ability to form tumors upon transplantation into the
fat pad of syngeneic hosts. |
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Keywords: | mammary in vitro carcinogenesis collagen |
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