Ca2+ antagonist-insensitive coronary smooth muscle contraction involves activation of epsilon-protein kinase C-dependent pathway |
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Authors: | Dallas Andrea Khalil Raouf A |
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Affiliation: | Harvard Medical School, VA Boston Healthcare-Research, 1400 VFW Parkway, 3/2B123, Boston, MA 02132, USA. |
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Abstract: | Certain angina and coronary artery disease forms do not respond to Ca2+ channel blockers, and a role for vasoactive eicosanoids such as PGF2 in Ca2+ antagonist-insensitive coronary vasospasm is suggested; however, the signaling mechanisms are unclear. We investigated whether PGF2 -induced coronary smooth muscle contraction is Ca2+ antagonist insensitive and involves activation of a PKC-dependent pathway. We measured contraction in single porcine coronary artery smooth muscle cells and intracellular free Ca2+ concentration ([Ca2+]i) in fura 2-loaded cells and examined cytosolic and particulate fractions for PKC activity and reactivity with isoform-specific PKC antibodies. In Hanks' solution (1 mM Ca2+), PGF2 (10-5 M) caused transient [Ca2+]i increase followed by maintained [Ca2+]i increase and 34% cell contraction. Ca2+ channel blockers verapamil and diltiazem (10-6 M) abolished maintained PGF2 -induced [Ca2+]i increase but only partially inhibited PGF2 -induced cell contraction to 17%. Verapamil-insensitive PGF2 contraction was inhibited by PKC inhibitors GF-109203X, calphostin C, and -PKC V1-2. PGF2 caused Ca2+-dependent -PKC and Ca2+-independent -PKC translocation from cytosolic to particulate fractions that was inhibited by calphostin C. Verapamil abolished PGF2 -induced -but not -PKC translocation. PMA (10-6 M), a direct activator of PKC, caused 21% contraction with no significant [Ca2+]i increase and -PKC translocation that were inhibited by calphostin C but not verapamil. Membrane depolarization by 51 mM KCl, which stimulates Ca2+ influx, caused 36% cell contraction and [Ca2+]i increase that were inhibited by verapamil but not GF-109203X or calphostin C and did not cause - or -PKC translocation. Thus a significant component of PGF2 -induced contraction of coronary smooth muscle is Ca2+ antagonist insensitive, involves Ca2+-independent -PKC activation and translocation, and may represent a signaling mechanism of Ca2+ antagonist-resistant coronary vasospasm. eicosanoids; calcium; vascular smooth muscle |
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