Brush border enzyme activities in the small intestine after long-term gliadin feeding in animal models of human c?liac disease |
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Authors: | H Kozáková R ?těpánková J Kolínská M A Farré D P Funda L Tu?ková H Tlaskalová-Hogenová |
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Institution: | (1) Division of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic;(2) Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic |
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Abstract: | Cœliac disease is a human, genetically linked, disorder which develops in gluten-sensitive persons. The aim of this study
was to investigate the effect of prolonged feeding of gliadin, a major fraction of gluten, on enzyme activities of enterocyte
brush border membrane enzymes in rats, mice and pigs. Brush-border membranes were isolated from mucosal scrapings of the small
intestine of 21-d-old rat pups hand-fed with formula milk diet, two-month-old nu/nu and +/+BALB/c mice and two-month-old piglets
fed three times a week starting at birth with high doses of gliadin. Activities of lactase, sucrase and dipeptidyl peptidase
IV (DPP IV) were determined. Individual animal models differed in their response to gliadin feeding. In comparison with albumin
fed controls the activities of DPP IV and lactase were decreased in rat pups, nu/nu BALB/c mice and piglets. DPP IV activity
was mostly affected in the ileum of rats and piglets fed with gliadin starting at birth. On the other hand, lactase and sucrase
activities of nu/nu BALB/c mice and piglets decreased to the largest extent in jejunum. |
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