| mRNA展示技术 |
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| 引用本文： | 张万巧,王 建,贺福初.mRNA展示技术[J].生物化学与生物物理进展,2006,33(8):795-799. |
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| 作者姓名： | 张万巧 王 建 贺福初 |
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| 作者单位： | 北京放射与辐射医学研究所,北京,100850 |
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| 基金项目： | 国家高技术研究发展计划(863计划);北京市科委科技计划项目 |
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| 摘 要： | mRNA展示技术是一种新兴的体外筛选多肽和蛋白质的有力工具.在筛选过程中,mRNA与其编码的多肽或蛋白质共价结合,形成mRNA-蛋白质融合体,能在大容量的多肽文库(1013～1015)中筛选具有特定生物学功能的多肽和蛋白质.目前,mRNA展示技术主要应用于各种靶分子的多肽和蛋白质适体的发现以及蛋白质相互作用机制的阐明和分析.由于其自身的巨大发展潜力,mRNA展示技术具有更为广阔的应用前景.
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| 关 键 词： | 体外展示技术 mRNA展示技术 mRNA-蛋白质融合体 |
| 收稿时间： | 3/30/2006 1:01:05 AM |
| 修稿时间： | 2006-03-302006-04-29 |
mRNA Display Technology |
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| ZHANG Wan-Qiao,WANG Jian and HE Fu-Chu.mRNA Display Technology[J].Progress In Biochemistry and Biophysics,2006,33(8):795-799. |
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| Authors: | ZHANG Wan-Qiao WANG Jian and HE Fu-Chu |
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| Institution: | Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract: | mRNA display provides a new powerful tool for in vitro selecting of peptides and proteins.In the selecting process,peptides are covalently attached to their own mRNA to form mRNA-protein fusions.These mRNA-protein fusions enable in vitro selection of peptide and protein libraries of more than 1013 different sequences.The experiment conditions and protocols have been optimized in recent years.The application of mRNA display technology is mainly in the discovery of ligands for many kinds of target molecules and the analysis and mechanism elucidation on interaction between proteins.With its great potential,there will be a wide application foreground in the application of mRNA display. |
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| Keywords: | in vitro display technology mRNA display technology mRNA-protein fusion |
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