Understanding Romanowsky staining |
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| Authors: | R W Horobin D Curtis L Pindar |
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| Institution: | (1) Department of Biomedical Science, University of Sheffield, S10 2TN Sheffield, England;(2) Department of Zoology, University of Sheffield, S10 2TN Sheffield, England;(3) Sub-Department of Medical Genetics, University of Sheffield, 117 Manchester Road, S10 5DN Sheffield, England |
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| Abstract: | Summary Romanowsky staining of suspension-fixed lymphocytes and fibroblasts, deposited as monolayers on slides, involves an initial basic dyeing process followed by formation of a hydrophobic Azur B/Eosin Y complex at the more permeable and so faster staining cellular sites. This mechanism is shared with blood and marrow smears. However certain morphological features peculiar to suspension-fixed, cell culture-derived preparations also influence the staining pattern via rate control: namely the irregular and bulky profiles of fibroblasts, compared to the smoother and thinner lymphocytes; and the occasional superficial occlusion of cells by culture medium. |
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