Light and molybdenum requirements for substrate induction of nitrate reductase in cotyledons of lettuce,Lactuca sativa L. |
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| Authors: | J S Knypl Krystyna M Janas |
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| Institution: | (1) Laboratory of Plant Growth Substances, University of Lodz, Ul. Banacha12/16, 90-237 Lodz, Poland |
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| Abstract: | Light was required for induction of nitrate reductase (NR, E.C. 1.6.6.1) in intact cotyledons of 2-day old seedlings ofLactuca sativa L. Molybdate strongly enhanced efficiency of induction. Benzyladenine (BA), gibberellin, and succinic acid-2,2-dimethylhydrazide
reduced the enzyme activity. BA thrice enhanced incorporation of labelled leucine to the protein fraction. (2-chloroethyl)trimethylammonium
chloride did not affect NR activity and markedly inhibited greening and protein synthesis. KNO3 stimulated protein synthesis as well as growth of the cotyledons. |
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