Biomechanical properties of murine TMJ articular disc and condyle cartilage via AFM-nanoindentation |
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| Affiliation: | 1. School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA 19104, United States;2. Department of Surgery, The Children’s Hospital of Philadelphia, Philadelphia, PA 19104, United States;3. Department of Orthodontics, University Hospital, University of Tübingen, Tübingen 72076, Germany;4. Department of Mechanical Engineering, University of Delaware, Newark, DE 19716, United States;1. University of Maryland Baltimore School of Medicine, Department of Neurology, 110 S. Paca St, Baltimore, MD 21202, USA;2. IRCCS Fondazione Don Carlo Gnocchi ONLUS, Biomedical Technology Department, via Capecelatro 66, 20148 Milano, Italy;3. Politecnico di Milano, Department of Electronics, Information and Bioengineering, via Golgi 39, 20133 Milano, Italy;1. INRIA Paris, 2 Rue Simone Iff, 75012 Paris, France;2. Sorbonne Universités, UPMC Univ. Paris 6, Laboratoire Jacques-Louis Lions, 75252 Paris, France;3. Medical R&D, WBL Healthcare, Air Liquide Santé International, 1 Chemin de la Porte des Loges, 78350 Les Loges-en-Josas, France;4. Department of Mechanical Engineering, Lafayette College, Easton, PA 18042, USA;1. Biomedical Engineering, University of Strathclyde, UK;2. Institute for Applied Health Research, Glasgow Caledonian University, UK;3. School of Health Sciences, University of Salford, UK;4. Stroke MCN, NHS Lanarkshire, UK;1. Department of Engineering, Faculty of Science and Technology, Aarhus University, Finlandsgade 22, 8200 Aarhus N, Denmark;2. Department of Cardiothoracic & Vascular Surgery, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark;3. Department of Clinical Medicine, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark |
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| Abstract: | This study aims to quantify the biomechanical properties of murine temporomandibular joint (TMJ) articular disc and condyle cartilage using AFM-nanoindentation. For skeletally mature, 3-month old mice, the surface of condyle cartilage was found to be significantly stiffer (306 ± 84 kPa, mean ± 95% CI) than those of the superior (85 ± 23 kPa) and inferior (45 ± 12 kPa) sides of the articular disc. On the disc surface, significant heterogeneity was also detected across multiple anatomical sites, with the posterior end being the stiffest and central region being the softest. Using SEM, this study also found that the surfaces of disc are composed of anteroposteriorly oriented collagen fibers, which are sporadically covered by thinner random fibrils. Such fibrous nature results in both an F-D3/2 indentation response, which is a typical Hertzian response for soft continuum tissue under a spherical tip, and a linear F-D response, which is typical for fibrous tissues, further signifying the high degree of tissue heterogeneity. In comparison, the surface of condyle cartilage is dominated by thinner, randomly oriented collagen fibrils, leading to Hertzian-dominated indentation responses. As the first biomechanical study of murine TMJ, this work will provide a basis for future investigations of TMJ tissue development and osteoarthritis in various murine TMJ models. |
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| Keywords: | Temporomandibular joint Murine models Fibrocartilage Nanoindentation Heterogeneity |
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