Influence of erythrocyte aggregation on radial migration of platelet-sized spherical particles in shear flow |
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| Affiliation: | 1. Laboratory of Biorheology and Medical Ultrasonics, University of Montreal Hospital Research Center (CRCHUM), Montréal, Québec, Canada;2. Institute of Biomedical Engineering, University of Montreal, Montréal, Québec, Canada;3. Department of Radiology, Radio-oncology and Nuclear Medicine, University of Montreal, Montréal, Québec, Canada;1. Division of Chemical Biology and Medicinal Chemistry, College of Pharmacy, University of Texas, Austin, TX 78712, United States;2. Department of Molecular Biosciences, University of Texas, Austin, TX 78712, United States;1. Key Laboratory of Medical Molecular Probes, Department of Chemistry, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, China;2. Department of Immunity, Xinxiang Medical University, Xinxiang, Henan 453003, China;3. School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, China;1. ‘Ilie Murgulescu’ Institute of Physical Chemistry of the Romanian Academy, Colloid Chemistry Laboratory, 202 Spl. Independentei, 060021 Bucharest, Romania;2. Department of Engineering of Polymer Materials, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, H. Sienkiewicza 112, 90-363 Lodz, Poland;1. Superresolution Microscopy, Institute of Molecular Biology (IMB), D-55128 Mainz, Germany;2. Physics Department, Mainz University (JGU), D-55128 Mainz, Germany;3. Kirchhoff Institute of Physics (KIP), Heidelberg University, D-69120 Heidelberg, Germany;4. Department of Cell Biophysics, Jagiellonian University, Krakow, Poland;5. Institute of Pharmacy & Molecular Biotechnology (IPMB), Heidelberg University, D-69120 Heidelberg, Germany |
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| Abstract: | Blood platelets when activated are involved in the mechanisms of hemostasis and thrombosis, and their migration toward injured vascular endothelium necessitates interaction with red blood cells (RBCs). Rheology co-factors such as a high hematocrit and a high shear rate are known to promote platelet mass transport toward the vessel wall. Hemodynamic conditions promoting RBC aggregation may also favor platelet migration, particularly in the venous system at low shear rates. The aim of this study was to confirm experimentally the impact of RBC aggregation on platelet-sized micro particle migration in a Couette flow apparatus. Biotin coated micro particles were mixed with saline or blood with different aggregation tendencies, at two shear rates of 2 and 10 s−1 and three hematocrits ranging from 20 to 60%. Streptavidin membranes were respectively positioned on the Couette static and rotating cylinders upon which the number of adhered fluorescent particles was quantified. The platelet-sized particle adhesion on both walls was progressively enhanced by increasing the hematocrit (p < 0.001), reducing the shear rate (p < 0.001), and rising the aggregation of RBCs (p < 0.001). Particle count was minimum on the stationary cylinder when suspended in saline at 2 s−1 (57 ± 33), and maximum on the rotating cylinder at 60% hematocrit, 2 s−1 and the maximum dextran-induced RBC aggregation (2840 ± 152). This fundamental study is confirming recent hypotheses on the role of RBC aggregation on venous thrombosis, and may guide molecular imaging protocols requiring injecting active labeled micro particles in the venous flow system to probe human diseases. |
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| Keywords: | Mimicking platelets Red blood cell aggregation Cell migration Biorheology Micro particle dynamics Couette flow experiments |
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