Reactivity improvement of cellulolytic enzyme lignin via mild hydrothermal modification |
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| Affiliation: | 1. Key Laboratory of Bio-based Material Science and Technology of Ministry of Education, Northeast Forestry University, Harbin, China;2. Fifth Oil Production Plant, Daqing Oilfield Limited Company, Daqing, China;1. Departamento de Física, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil;2. Centro de Microscopia da Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil;1. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, PR China;2. College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510640, PR China;3. The State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China;1. Department of Process Engineering, Stellenbosch University, South Africa;2. Department of Forestry and Wood Sciences, Stellenbosch University, South Africa;1. School of Energy and Power Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;2. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, China |
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| Abstract: | Isolated by the cellulolytic enzyme lignin (CEL) process, water-alcohol (1:1, v/v) was introduced as co-solvent in the process of the hydrothermal treatment. The modification parameters such as reaction temperature and time, solid-to-liquid ratio, and catalysts (NaOH and NaOAlO2) have been investigated in terms of the specific lignin properties, such as the phenolic hydroxyl content (OHphen), DPPH free radical scavenging rate, and formaldehyde value. The CELs were also characterized by GPC, FT-IR and 1 H NMR spectroscopy, and Py-GC/MS. The key data are under optimal lignin modification conditions (solid-to-liquid ratio of 1:10 (w/v) and a temperature of 250 °C for 60 min) are: OHphen content: 2.50 mmol/g; half maximal inhibitory concentration (IC50) towards DPPH free radicals: 88.2 mg/L; formaldehyde value: 446.9 g/kg). Both base catalysts decrease the residue rate, but phenol reactivities of the products were also detracted. Py-GC/MS results revealed that modified lignin had a higher phenolic composition than the CEL did, especially the modified lignin without catalyst (ML), which represented 74.51% phenolic content. |
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| Keywords: | Cellulolytic enzyme lignin Characterization Hydrothermal modification Phenol reactivity Water-alcohol co-solvent |
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