B2-like repetitive sequence from the X Chromosome of the American mink (Mustela vison) |
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Authors: | Marina V Lavrentieva Mark I Rivkin Alexander G Shilov Marina L Kobetz Igor B Rogozin Oleg L Serov |
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Institution: | (1) Siberian Department, Institute of Cytology and Genetics, Academy of Sciences of the USSR, Novosibirsk-90, USSR |
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Abstract: | In analysis of the repeats from the mink X Chromosome (Chr), we have identified a B2-like repetitive sequence of 195 base pairs (bp) flanked by short direct repeats of 14 bp. It contains regions homologous to the split intragenic RNA polymerase III promoter and a 3 A-rich region followed by an oligo(dA) sequence. A feature of the repeat is the presence of a perfect polypyrimidine tract 22 bp in length absent from the known Alu- and Alu-like sequences. Alignment of the mink B2-like sequence and mouse B2-consensus sequence allowed us to estimate their similarity as 55%. The repeat is present in 1–2×105 copies per mink genome and 2–4×103 copies per X Chr. In situ hybridization analysis demonstrated a similar distribution pattern of the B2-like repeat along the length of all the mink chromosomes including the X. We also observed the presence of mink B2-like hybridizable sequence in the genomes of other Carnivora species.The nucleotide sequence data reported in this paper have been submitted to EMBL Data Library and have been assigned the accession number X52381 (MVB2RPT). |
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