Partial characterization of neural-inducing factors from Xenopus gastrulae Evidence for a larger protein complex containing the factor |
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Authors: | Jutta Janeczek Jochen Born Peter Hoppe Hildegard Tiedemann |
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Institution: | (1) Institut fur Molekularbiologie and Biochemie, Freie Universität Berlin, Arnimallee 22, W-1000 Berlin 33, Germany |
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Abstract: | Summary High (Mr 90–110 kDa) and low (Mr 15–30 kDa) molecular weight forms of neural-inducing factors have been found in the supernatant of Xenopus gastrula homogenate. The factors, which are protein in nature, have been partially purified by size exclusion high-performance liquid chromatography (HPLC) and sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. The factor of smaller size, which could be derived from a precursor, is associated with other proteins in a larger complex. The neural-inducing factors are not irreversibly inactivated after chemical deglycosylation with trifluoromethansulfonic acid. The neural-inducing protein which is found in ribonucleoprotein (RNP)-particles was partially purified by hydrophobic chromatography. Possible relationships of the factors in different subcellular fractions and their physiological significance are discussed.
Offprint requests to: H. Tiedemann |
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Keywords: | Neural induction Protein factors Xenopus laevis |
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