| 双歧杆菌表达ETEC CFA／I的免疫电镜定位分析 |
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| 引用本文： | 刘革力,冉丹霞,王荣荣,郝亚宁,宋方洲,马永平.双歧杆菌表达ETEC CFA／I的免疫电镜定位分析[J].中国微生态学杂志,2009,21(5):415-416. |
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| 作者姓名： | 刘革力 冉丹霞 王荣荣 郝亚宁 宋方洲 马永平 |
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| 作者单位： | 重庆医科大学,生物化学与分子生物学教研室,分子医学与肿瘤研究中心,重庆,400016 |
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| 摘 要： | 目的研究双歧杆菌表达的CFA／I在细胞中的分布。方法将培养8～10h的重组双歧杆菌固定后制作电镜切片，镜检合格后用镍网捞取3-5个切片与金标抗体作用，镜检CFA／I在双歧杆菌中的表达分布。结果自制的胶体金颗粒均匀，与抗体结合后能够示踪CFA／I在双歧杆菌中的表达分布。结论双歧杆菌表达的CFA／I主要集中在靠近细胞膜的周质腔一侧，利于向外分泌和释放。
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| 关 键 词： | 双歧杆菌 口服疫苗 CFA/I 免疫电镜 |
Analysis of ETEC CFA/I expressed in bifidobacterium by immuno-electron microscope |
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| LIU Ge-li,RAN Dan-xia,WANG Rong-rong,HAO Ya-ning,SONG Fang-zhou,MA Yong-ping.Analysis of ETEC CFA/I expressed in bifidobacterium by immuno-electron microscope[J].Chinese Journal of Microecology,2009,21(5):415-416. |
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| Authors: | LIU Ge-li RAN Dan-xia WANG Rong-rong HAO Ya-ning SONG Fang-zhou MA Yong-ping |
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| Institution: | (Department of Biochemistry and Molecular Biology,Molecular Medical and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China) |
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| Abstract: | Objective To determine CFA/I expression area in bifutobacterium. Method After cultured 8 - 10 hours, the section of recombinant bifidobacterium was prepared for electron microscopy and then for reaction with golden labeled anti-CFA/I antibody. The resulted products were examined with H-7500 electron microscope. Result The golden la- beled antibody could react with CFA/I expressed in bifidobacterium. Conclusion Golden labeled CFA/I was found in the area of periplasmic space,which was favorable to CFA/I excretion as an oral live vaccine antigen. |
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| Keywords: | CFA/I |
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