| 用阳离子交换树脂测定尿三甲基组氨含量 |
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| 引用本文： | 黎君友.用阳离子交换树脂测定尿三甲基组氨含量[J].氨基酸和生物资源,1992(3):49-52. |
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| 作者姓名： | 黎君友 |
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| 作者单位： | 304医院创伤中心外科研究室 |
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| 摘 要： | 因3-甲基组氨酸(3MH)从肌肉蛋白降解后,不重新参加蛋白质的合成,而从尿中排出,所以把它作为肌肉蛋白质分解的可靠指标。本文以Radha和Bess-man在1982年建立,Fitch等在1986年改进的阳离子交换树脂法。以大部国产试剂,对柱长、内径、pH等作了适当的改进,得到了满意的测定3MH的结果。从0.15到1.0μMol的三点标准曲线与Fitch等的相似,此法重复性好,回收率高,测定范围广,是较为简单,可推行的方法。用此法测定了烧伤和正常鼠尿3MH的含量,烧伤组14.5%Cv和对照组13.0%Cv,显示大鼠每天尿中排出的3MH含量比较稳定,说明烧伤和正常鼠肌肉蛋白质的分解比较恒定。烧伤组鼠尿3MH的每天排出量高于正常组35%以上,表明烧伤鼠肌肉蛋白质的分解速率高于对照组。
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| 关 键 词： | 阳离子交换树脂 三甲基组氨酸 烧伤鼠 尿 |
Measurement of urinary 3-methylhistidine with cationic-exchange resin |
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| Institution: | ,Li jun-you Trauma center. postgraduate medical college and 304th hospital of PLA |
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| Abstract: | Measurement of urinary 3-methylhistidine (3 MH) with cation exch-
ange resin suggested that this method had many advantages, such as high
repetition, high rate of collection, wide range of measurement and simp-
lification.
Our results showed that content of 3 MH in burned rat was 35% higher
than that of normal rat, indicating an increased of proteolysis in muscle
after burn. |
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