Complete microscale profiling of tumor microangiogenesis: A microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterization |
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| Authors: | Chia-Chi Chien Ivan M Kempson Cheng Liang Wang HH Chen Yeukuang Hwu NY Chen TK Lee Kelvin K-C Tsai Ming-Sheng Liu Kwang-Yu Chang CS Yang G Margaritondo |
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| Institution: | 1. Institute of Physics, Academia Sinica, Nankang, Taipei 115, Taiwan;2. Department of Engineering and System Science, National Tsing Hua University, Hsinchu 300, Taiwan;3. Institute of Optoelectronics Sciences, National Taiwan Ocean University, Keelung 202, Taiwan;4. National Center for High-performance Computing, Hsinchu 300, Taiwan;5. National Institute of Cancer Research and Translational Center for Glandular Malignancies, National Health Research Institutes, Tainan 704, Taiwan;6. Center for Nanomedicine, National Health Research Institutes, Miaoli 350, Taiwan;g Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland |
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| Abstract: | Complete profiling would substantially facilitate the fundamental understanding of tumor angiogenesis and of possible anti-angiogenesis cancer treatments. We developed an integrated synchrotron-based methodology with excellent performances: detection of very small vessels by high spatial resolution (~ 1 μm) and nanoparticle contrast enhancement, in vivo dynamics investigations with high temporal resolution (~ 1 ms), and three-dimensional quantitative morphology parametrization by computer tracing. The smallest (3–10 μm) microvessels were found to constitute > 80% of the tumor vasculature and exhibit many structural anomalies. Practical applications are presented, including vessel microanalysis in xenografted tumors, monitoring the effects of anti-angiogenetic agents and in vivo detection of tumor vascular rheological properties. |
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| Keywords: | Angiogenesis Synchrotron X-ray imaging Solid tumor Quantitative analysis |
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