Recent advances in bioprocessing application of membrane chromatography |
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| Authors: | Valerie Orr Luyang ZhongMurray Moo-Young C. Perry Chou |
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| Affiliation: | Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1 |
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| Abstract: | Compared to traditional chromatography using resins in packed-bed columns, membrane chromatography is a relatively new and immature bioseparation technology based on the integration of membrane filtration and liquid chromatography into a single-stage operation. Over the past decades, advances in membrane chemistry have yielded novel membrane devices with high binding capacities and improved mass transfer properties, significantly increasing the bioprocessing efficiency for purification of biomolecules. Due to the disposable nature, low buffer consumption, and reduced equipment costs, membrane chromatography can significantly reduce downstream bioprocessing costs. In this review, we discuss technological merits and disadvantages associated with membrane chromatography as well as recent bioseparation applications with a particular attention on purification of large biomolecules. |
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| Keywords: | AcMNVP, Autographa californica multicapsid nucleopolyhedrovirus AeDNV, Aedes aegypti densonucleosis virus AGM, agmatine BSA, bovine serum albumin BUDGE, 1,4-butanediol diglycidyl ether C, carboxyl weak cation-exchanger CHO, Chinese hamster ovarian cells CM, carboxyl methyl weak cation-exchanger DBC, dynamic binding capacity DEA, diethyl amine weak anion-exchanger DEAE, diethylaminoethyl weak anion-exchanger EEL, Euonymus europaeus lectin EU, endotoxin unit GFP, green fluorescent protein GMA, glycidyl methacrylate HA, hemoagglutinin HAU, hemoagglutinin units HCP, host cell protein HMW, high molecular weight IDA, iminodiacetic acid IgG, immunoglobulin L-DOPA, 3, 4-dihydroxy-phenylalanine LRV, log reduction value MAB, monoclonal antibody MVM, minute viruses of mouse NTA, nitrilotriacetic acid PAA, polyallylamine pDNA, plasmid DNA PEI, polyethyleneimine PES, polyethersulfone PFU, plaque forming units PHMB, polyhexamethylene biguanide pI, isoelectric point PP, polypropylene PPV, parvovirus PVDF, polyvinylidene fluoride Q, quaternary amine strong anion-exchanger RC, regenerated cellulose RLP, rotavirus like particle S, sulfonated strong cation-exchanger SBC, static binding capacity TAEA, tris-2-aminoethyl amine TCID50, 50% tissue culture infective dose TFF, tangential flow filtration |
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