Advantages of intermediate X-ray energies in Zernike phase contrast X-ray microscopy |
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| Authors: | Zhili Wang Kun Gao Jian Chen Youli Hong Xin Ge Dajiang Wang Zhiyun Pan Peiping Zhu Wenbing Yun Chris Jacobsen Ziyu Wu |
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| Institution: | 1. National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230026, China;2. Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;3. Xradia Inc., 4385 Hopyard Road, Pleasanton, CA 94588, USA;4. Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA;5. Northwestern University, Evanston, IL, USA |
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| Abstract: | Understanding the hierarchical organizations of molecules and organelles within the interior of large eukaryotic cells is a challenge of fundamental interest in cell biology. Light microscopy is a powerful tool for observations of the dynamics of live cells, its resolution attainable is limited and insufficient. While electron microscopy can produce images with astonishing resolution and clarity of ultra-thin (< 1 μm thick) sections of biological specimens, many questions involve the three-dimensional organization of a cell or the interconnectivity of cells. X-ray microscopy offers superior imaging resolution compared to light microscopy, and unique capability of nondestructive three-dimensional imaging of hydrated unstained biological cells, complementary to existing light and electron microscopy. |
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| Keywords: | X-ray microscopy Zernike phase contrast Intermediate energy Tomography Radiation dose Depth-of-focus |
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