Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution |
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| Authors: | Sylvain Clè de,Franç ois Lambert,Christophe Sandt,Zoher Gueroui,Nicolas Delsuc,Paul Dumas,Anne Vessiè res,Clotilde Policar |
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| Affiliation: | 1. Ecole Normale Supérieure, Département de chimie, 24, rue Lhomond, 75005 Paris, France;2. Université Pierre et Marie Curie Paris 6, 4, Place Jussieu, 75005 Paris, France;3. CNRS, UMR7203, France;4. CNRS, UMR8640, France;5. SMIS Synchrotron SOLEIL Saint-Aubin, 91192, Gif-sur-Yvette Cedex, France;6. Laboratoire Friedel, Chimie ParisTech, CNRS, UMR7223, 11, rue Pierre et Marie Curie, 75231 Paris, France |
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| Abstract: | 1,1-Di(4-hydroxyphenyl)-2-cyrhetrenylbut-1-ene 1 is an organometallic conjugate where a [(Cp)Re(CO)3] unit is linked to a hydroxytamoxifen-like structure. Its subcellular nuclear distribution was previously observed in a single cell using the near-field technique AFMIR. We show here that synchrotron radiation FTIR spectromicroscopy (SR-FTIR-SM) enabled the mapping of 1 based on its IR-signature (characteristic bands in the 1850–2200 cm− 1 range) and pointed out the colocalization of 1 with an area of high amide density. Fluorescence microscopy using DAPI staining performed on the same cells confirmed that this area corresponds to the cell nucleus. |
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| Keywords: | Correlative spectroscopies Infrared spectromicroscopy Fluorescence microscopy Subcellular imaging Organometallic probe Rhenium tris-carbonyl |
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