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Comparison of Methods for DNA Isolation from Food Samples for Detection of Shiga Toxin-Producing Escherichia coli by Real-Time PCR |
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| Authors: | Loree C Heller Carisa R Davis K Kealy Peak David Wingfield Andrew C Cannons Philip T Amuso and Jacqueline Cattani |
| Abstract: | In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly. |
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