Current status of antisense DNA methods in behavioral studies |
| |
Authors: | Ogawa S; Pfaff DW |
| |
Institution: | Laboratory of Neurobiology and Behavior, Rockefeller University, New York, NY 10021, USA. ogawa@rockvax.rockefeller.edu |
| |
Abstract: | The antisense DNA method has been used successfully to block the expression
of specific genes in vivo in neuronal systems. An increasing number of
studies in the last few years have shown that antisense DNA administered
directly into the brain can modify various kinds of behaviors. These
findings strongly suggest that the antisense DNA method can be used as a
powerful tool to study causal relationships between molecular processes in
the brain and behavior. In this article we review the current status of the
antisense method in behavioral studies and discuss its potentials and
problems by focusing on the following four aspects; (i) optimal application
paradigms of antisense DNA methods in behavioral studies; (ii) efficiencies
of different administration methods of antisense DNA used in behavioral
studies; (iii) determination of specificity of behavioral effects of
antisense DNA; and (iv) discrepancies between antisense DNA effects on
behaviors and those on protein levels of the targeted gene.
|
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|