Long-Range 1H-15N Heteronuclear Shift Correlation at Natural Abundance: a Tool To Study Benzothiazole Biodegradation by Two Rhodococcus Strains |
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| Authors: | Pascale Besse, Bruno Combourieu, Gaë lle Boyse, Martine Sancelme, Heleen De Wever, Anne-Marie Delort |
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| Affiliation: | Pascale Besse, Bruno Combourieu, Gaëlle Boyse, Martine Sancelme, Heleen De Wever, and Anne-Marie Delort |
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| Abstract: | The biodegradation of benzothiazole and 2-hydroxybenzothiazole by two strains of Rhodococcus was monitored by reversed phase high-pressure liquid chromatography and by 1H nuclear magnetic resonance (NMR). Both xenobiotics were biotransformed into a hydroxylated derivative of 2-hydroxybenzothiazole by these two strains. The chemical structure of this metabolite was determined by a new NMR methodology: long-range 1H-15N heteronuclear shift correlation without any previous 15N enrichment of the compound. This powerful NMR tool allowed us to assign the metabolite structure to 2,6-dihydroxybenzothiazole. |
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