Technique for measuring picomolar amounts of bound and unbound amino acids obtained from myogenic cell cultures of skeletal muscle |
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Authors: | Joseph C Bullaro |
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Institution: | (1) Department of Anatomy, University of Saskatchewan, S7N 0W0 Saskatoon, Saskatchewan, Canada |
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Abstract: | Summary A procedure is described that compares the isotope dilution method of measuring picomolar amounts of amino acids obtained
from cellular extracts with a direct method of analysis. Evidence is provided that shows that the direct method is at least
as accurate as the isotope dilution method. In addition the direct method is as expedient and requires but a single isotope
and fewer chromatograms for analysis. A procedure also is described for selecting the appropriate conditions for dansylation
and for measuring the loss of dansyl amino acid due to decomposition.
This research was funded through a grant from the Muscular Dystrophy Association of Canada. |
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Keywords: | amino acids ultra micromethod myogenic cell culture |
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