The carboxy-terminal extension of the D1-precursor protein is dispensable for a functional photosystem II complex in Chlamydomonas reinhardtii |
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Authors: | Silke Schrader Udo Johanningmeier |
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Institution: | (1) Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität Bochum, Postfach 1021 48, 4630 Bochum 1, Germany |
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Abstract: | The D1-precursor protein of the photosystem II reaction centre contains a carboxy-terminal extension whose proteolytic removal is necessary for oxygen-evolving activity. To address the question of the role of the carboxy-terminal extension in the green alga Chlamydomonas reinhardtii, we truncated D1 by converting codon Ser345 of the psbA gene into a stop codon. Particle gun transformation of an in vitro modified psbA gene fragment also carrying mutations conferring herbicide resistance yielded a homoplasmic transformant containing the stop codon. Since oxygen evolution capacity is not affected in this mutant as compared with herbicide-resistant control cells, the carboxy-terminal extension is dispensable for a functional photosystem II complex under normal growth conditions. |
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Keywords: | Chlamydomonas reinhardtii D1 carboxy terminus photosystem II chloroplast transformation |
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