Genetic architecture of fear conditioning in chromosome substitution strains: relationship to measures of innate (unlearned) anxiety-like behavior |
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Authors: | Christine A Ponder Michaelanne Munoz T Conrad Gilliam Abraham A Palmer |
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Institution: | (1) Department of Genetics and Development, Columbia University, New York, New York, USA;(2) Department of Human Genetics, University of Chicago, Chicago, Illinois, USA;(3) Department of Human Genetics, University of Chicago, 920 E. 58th St. CLSC 507D, Chicago, IL 60637, USA |
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Abstract: | We measured fear conditioning (FC) in a panel of chromosome substitution strains (CSS) created using the C57BL/6J (B6) and
A/J (AJ) inbred strains. Mice were trained to associate a specific context and tone with a foot shock. FC was measured by
observing freezing behavior during re-exposure to the context and tone. Freezing to context was more than twofold greater
in the AJ strain relative to the B6 strain. Among the CSS we identified four strains with higher (CSS-6, -10, -11, and -18)
and two strains with lower (CSS-7 and -14) freezing to context. CSS-10 and -18 also showed higher freezing to tone, while
CSS-12 showed less freezing to tone. CSS-1 has been implicated in open-field (OF) and light-dark box (LDB); we observed significant
activity differences prior to training but no differences in FC. Chromosomes 6 and 10 have been associated with differences
in anxiety-like behaviors, suggesting the existence of pleiotropic alleles that influence both learned and innate fear. By
utilizing a genetic reference population, we have identified chromosomes that pleiotropically influence multiple phenotypes
hypothesized to reflect a common ethologic construct that has been termed emotionality. The CSS provide a straightforward
means of isolating the underlying genetic factors. |
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