Characterization of the Human Dihydropyrimidine Dehydrogenase Gene |
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Authors: | Xiaoxiong Wei Guillermo Elizondo Andrea Sapone Howard L. McLeod Hannu Raunio Pedro Fernandez-Salguero Frank J. Gonzalez |
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Affiliation: | aLaboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20892;bDepartment of Medicine and Therapeutics, University of Aberdeen, Aberdeen, AB9 2ZD, United Kingdom;cDepartment of Pharmacology and Toxicology, University of Oulu, Oulu, Finland |
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Abstract: | Dihydropyrimidine dehydrogenase (DPD) catabolizes endogenous pyrimidines and pyrimidine-based antimetabolite drugs. A deficiency in human DPD is associated with congenital thymine-uraciluria in pediatric patients and severe 5-fluorouracil toxicity in cancer patients. The dihydropyrimidine dehydrogenase gene (DPYD) was isolated, and its physical map and exon–intron organization were determined by analysis of P1, PAC, BAC, and YAC clones. TheDPYDgene was found to contain 23 exons ranging in size from 69 bp (exon 15) to 961 bp (exon 23). A physical map derived from a YAC clone indicated thatDPYDis at least 950 kb in length with 3 kb of coding sequence and an average intron size of about 43 kb.The previously reported 5′ donor splice site mutation present in pediatric thymine-uraciluria and cancer patients can now be assigned to exon 14. All 23 exons were sequenced from a series of human DNA samples, and three point mutations were identified in three racial groups as G1601A (exon 13, Ser534Asn), A1627G (exon 13, Ile543Val), and G2194A (exon 18, Val732Ile). These studies, which have established that theDPYDgene is unusually large, lay a framework for uncovering new mutations that are responsible for thymine-uraciluria and toxicity to fluoropyrimidine drugs. |
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