Production of C20 polyunsaturated fatty acids (PUFAs) by pathway engineering: identification of a PUFA elongase component from Caenorhabditis elegans |
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| Authors: | Beaudoin F Michaelson L V Lewis M J Shewry P R Sayanova O Napier J A |
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| Affiliation: | IACR-Long Ashton Research Station, Long Ashton, Bristol BS41 9AF, UK. |
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| Abstract: | Using a combination of database-mining and functional characterization, we have identified a component of the polyunsaturated fatty acid (PUFA) elongase. Co-expression of this elongating activity with fatty acid desaturases has allowed us to heterologously reconstitute the PUFA biosynthetic pathway. Both these enzymes (desaturases and elongase components) have undergone gene-duplication events which provide a paradigm for the diverged nature of PUFA biosynthetic activities. |
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