Evidence for type II phytochrome-induced rapid signalling leading to cab::luciferase gene expression in tobacco cotyledons |
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Authors: | Ingeborg Schütz Masaki Furuya |
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Institution: | (1) Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan, JP;(2) Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Carl-von-Linné-Weg 10, 50829 Cologne, Germany, DE |
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Abstract: | We studied the activation of cab gene expression by phytochrome-induced intercellular signalling and report insights into the mechanism of induction and outspread
of a plant internal signal. By micro-beam irradiation techniques and use of a photon-imaging charge-coupled device (CCD) camera
system we monitored cab::luciferase reporter gene expression in cotyledons of transgenic tobacco (Nicotiana tabacum L. cv. Xanthi) plants. We found that (i) the photoreceptor triggering intercellular signalling and reporter-gene expression
is type II but not type I phytochrome, (ii) phytochrome in its far-red-absorbing (Pfr) form is necessary for the induction
but not for the outspread of the signalling, (iii) red/far-red reversibility is restricted to the red-irradiated cells, and
(iv) the phytochrome-induced signal spreads rapidly throughout the cotyledon and reaches its target cells within minutes.
Received: 26 June 2000 / Accepted: 25 August 2000 |
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Keywords: | :cab::luciferase gene expression Micro-beam irradiation Nicotiana (transgenic) Photon imaging Phytochrome Signalling (rapid) |
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