Characterization of conjugative plasmids carrying antibiotic resistance genes encoding 16S rRNA methylase, extended-spectrum beta-lactamase, and/or plasmid-mediated AmpC beta-lactamase |
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Authors: | Hee Young Kang Jungmin Kim Sung Yong Seol Yoo Chul Lee Je Chul Lee Dong Taek Cho |
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Institution: | (1) Department of Microbiology, Kyungpook National University, School of Medicine, Daegu, 700-422, Republic of Korea |
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Abstract: | In this study, we identified extended-spectrum β-lactamase (ESBL) and plasmid-mediated AmpC β-lactamase which were associated
with 16S rRNA methylase gene on the conjugative plasmid. Among 82 clinical isolates of Enterobacteriaceae that carry 16S rRNA methylase gene (64 strains, armA, and 18 strains, rmtB), bla
SHV-12 was detected either alone or combined with bla
DHA-1, bla
CTX-M-3, and bla
CTX-M-14 in 30 strains carrying armA and 6 strains carrying rmtB. The bla
CTX-M-3 was detected in 13 of 64 strains carrying armA but no strains carrying rmtB. Whereas bla
CTX-M-14 was detected in 15 of 18 strains carrying rmtB but only 2 of 64 strains carrying armA. Overall, bla
SHV-12 and bla
CTX-M-14 was the most common ESBL gene which was associated with armA and rmtB, respectively. In addition, we found that bla
CTX-M-3 localized with armA on the same IncL/M plasmid and bla
CTX-M-14 localized with rmtB on the same IncA/C plasmid. Restriction fragment length polymorphism of conjugative plasmids and pulsed-field gel electrophoresis
of genomic DNAs revealed that intercellular horizontal transfer of conjugative plasmid and clonal transmission have been occurred
at the same time. |
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Keywords: | 16S rRNA methylase extended-spectrum beta-lactamase IncL/M IncA/C Enterobacteriaceae |
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