A simple structured model for biomass and extracellular enzyme production with recombinant Saccharomyces cerevisiae YPB-G |
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Authors: | Birol G Kirdar B Onsan Z I |
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Institution: | Department of Chemical Engineering, Bogazi?i University, 80815 Bebek, Istanbul, Turkey. |
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Abstract: | A simple structured model is proposed for simulating batch cultivation data on growth, substrate utilization, and heterologous
enzyme production of recombinant Saccharomyces cerevisiae YPB-G. The enzyme is a fusion protein displaying α-amylase and glucoamylase activities. Cell growth is modulated mainly by
intracellular substrate and ethanol concentrations. Intracellular substrate concentration is evaluated by means of the extracellular
substrate and biomass concentrations. Extracellular α-amylase and glucoamylase activities are taken to depend on biomass concentration.
The nine parameters of the proposed model are determined using nonlinear estimation techniques, and the model is validated
against experiments not used in parameter determination. The model developed simulates glucose consumption, cell mass, α-amylase
and glucoamylase production in a batch system. Simulation and experimental results are found to be in good agreement. Journal of Industrial Microbiology & Biotechnology (2002) 29, 111–116 doi:10.1038/sj.jim.7000281
Received 07 January 2002/ Accepted in revised form 22 May 2002 |
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Keywords: | : recombinant yeast structured modeling α -amylase glucoamylase batch cultivation |
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