Agrobacterium rhizogenes transformation of the Phaseolus spp.: a tool for functional genomics |
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Authors: | Estrada-Navarrete Georgina Alvarado-Affantranger Xochitl Olivares Juan-Elías Díaz-Camino Claudia Santana Olivia Murillo Enrique Guillén Gabriel Sánchez-Guevara Nayeli Acosta Jorge Quinto Carmen Li Dongxue Gresshoff Peter M Sánchez Federico |
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Institution: | Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62271, México. |
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Abstract: | A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, landraces, and cultivars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. Induced hairy roots are robust and grow quickly. The transformation frequency is between 75 and 90% based on the 35-S promoter-driven green fluorescent protein and beta-glucuronidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal determinate nodules that fix nitrogen as efficiently as inoculated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiology, root metabolism, and root-microbe interactions. |
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