Genetic identification of fish eggs collected in Sendai Bay and off Johban,Japan |
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| Authors: | Kenji Saitoh Shinji Uehara Taro Tega |
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| Affiliation: | (1) Tohoku National Fisheries Research Institute, Fisheries Research Agency, 3-27-5 Shinhama, Shiogama Miyagi, 985-0001, Japan;(2) Laboratory of Population Genetic Informatics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiya, Aoba, Sendai Miyagi, 981-8555, Japan;(3) Present address: National Research Institute of Fisheries Science, Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa, Yokohama Kanagawa, 236-8648, Japan;(4) Present address: IDEA Consultants Inc., Nagoya Office, 1-7-15 Irifune, Minato, Nagoya Aichi, 455-0032, Japan |
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| Abstract: | Mitochondrial DNA typing of 52 fish eggs collected in Sendai Bay and off Johban revealed ten haplotypes of the 225–251 bp region of 16S ribosomal DNA. Comparing with known sequences of fishes, we identified them as Trachurus japonicus (Carangidae), Pagrus major, Evynnis japonica (Sparidae), Paralichthys olivaceus, Pseudorhombus sp. (Paralichthidae), Pennahia argentata (Sciaenidae) and Platycephalus sp. (Platycephalidae). The occurrence of Trachurus japonicus and sparid eggs in the study area indicates significant extensions to the ranges of their spawning sites northward. Our method is useful for identifying fish eggs that are difficult to diagnose by morphology, and to uncover cryptic fish egg assemblages in the field. |
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| Keywords: | DNA typing Trachurus japonicus Sparidae Paralichthidae Pennahia argentata |
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