Ascorbic acid enhancement of organogenesis in tobacco callus |
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| Authors: | Richard W. Joy IV Kamlesh R. Patel Trevor A. Thorpe |
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| Affiliation: | (1) Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, T2N 1N4 Calgary, Alberta, Canada;(2) Present address: AgriForest Technologies Ltd, P.O. Box 178, V1Y 7N5 Kelowna, BC, Canada |
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| Abstract: | The effect of ascorbic acid on growth and shoot formation in callus cultures of tobacco (Nicotiana tabacum L.) was investigated, using young (4–12 subcultures) and old (more than 30 subcultures) tissue. It was found that ascorbate, at levels of 4–8×10-4M, enhanced shoot formation in both young and old callus. Treatment with ascorbate also speeded up the shoot-forming process. In addition, ascorbate completely reversed the inhibition of shoot formation by gibberellic acid in young callus, but was less effective in old callus. |
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| Keywords: | Nicotiana tabacum ascorbate callus cultures organogenesis shoot formation tobacco young and old callus |
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