Analysis of lymphocytes reactive to histocompatibility antigens. I. A quantitative titration assay for mixed lymphocyte interactions in the rat. |
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| Authors: | D F Antczak D Brown J C Howard |
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| Affiliation: | 2. Agricultural Research Council, Institute of Animal Physiology, Babraham, Cambridge CB2 4AT, U.K.;1. Agricultural Research Council, Statistics Group, Department of Applied Biology, University of Cambridge, U.K. |
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| Abstract: | This paper describes a method for quantitating relative potencies of mixed lymphocyte interactions (MLI) in the rat by parallel line analysis of responder cell titrations. The MLI titration assay is based upon, and has certain advantages over, the popliteal lymph node graft-versus-host assay for investigations of allogeneic interactions determined by the major histocompatibility complex. Log-log plots of dose of responder cells versus cpm of [3H]thymidine incorporated were linear in the range 1 × 104 to 2 × 105 responder cells per microtiter well. Dilution experiments indicated that the overriding influence on the potency of any allogeneic interaction was the initial frequency of reactive cells. We have defined the limits within which the MLI titration assay behaved ideally and suggested explanations for the nonideal behavior seen when reactions of widely differing potency were compared. |
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