Scanning electron microscopy of desiccation-tolerant and -sensitive microspore-derived embryos of Brassica napus L. |
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Authors: | K Wakui Y Takahata N Kaizuma |
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Institution: | (1) Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan Tel.: 81-19-621-6151 Fax: 81-19-621-6177 e-mail: ytakahata@iwate-u.ac.jp, JP |
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Abstract: | Desiccation tolerance can be induced in microspore-derived embryos of Brassica napus L. by application of abscisic acid (ABA). Scanning electron microscopy was employed to study and compare desiccation-tolerant
and -sensitive microspore-derived embryos. The external surface of those desiccated embryos in which desiccation tolerance
had been induced was uniformly shriveled due to severe dehydration, and their internal tissue system was well preserved. In
contrast, in desiccation-sensitive embryos, dehydration caused tearing of the epidermis and collapse of the internal tissue
system. After the desiccated embryos had been rehydrated, the size and external morphology of the desiccation-tolerant ones
recovered to the pre-desiccation state within 1 day, whereas the sensitive ones did not recover or remained shriveled. The
effect of ABA on the induction of desiccation tolerance is discussed.
Received: 25 June 1998 / Revision received: 03 September 1998 / Accepted: 24 September 1998 |
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Keywords: | Brassica napus L Abscisic acid Desiccation tolerance Microspore-derived embryo Scanning electron microscopy |
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