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Microsatellite loci for population and parentage analysis in the Amazon River dolphin (Inia geoffrensis de Blainville, 1817) |
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| Authors: | Gravena Waleska Hrbek Tomas DA Silva Vera M S Astolfi-Filho Spartaco Farias Izeni P |
| Institution: | 1. Laboratório de Evolu??o e Genética Animal, Departamento de Biologia, Universidade Federal do Amazonas, Av. Rodrigo Octávio Jord?o Ramos, 3000, 69077‐000, Manaus, AM, Brazil;2. Biology Department, University of Puerto Rico 3. — 4. Rio Piedras, San Juan, PR 00931, Puerto Rico;5. Laboratório de Mamíferos Aquáticos 6. CPBA 7. INPA, Manaus, Amazonas, Brazil;8. Laboratório de Tecnologias de DNA, Centro de Apoio Muldisciplinar, Universidade Federal do Amazonas, Av. Rodrigo Octávio Jord?o Ramos, 3000, 69077‐000, Manaus, AM, Brazil |
| Abstract: | We developed specific primers for microsatellite DNA regions for the Amazon River dolphin or boto Inia geoffrensis, for use in population and conservation genetic studies. We also tested their transferability for two other species, Pontoporia blainvillei (sister taxon of I. geoffrensis) and Sotalia guianensis. A total of 12 microsatellite loci were polymorphic for the boto. An additional 25 microsatellite loci previously isolated from other cetacean species were also tested in the boto. The 26 polymorphic microsatellite loci indicate they will be excellent markers for studies of population structure and kinship relations of the boto. |
| Keywords: | Amazon River dolphin boto‐rosa microsatellites Pontoporia blainvillei Sotalia guianensis |
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