Isolation and characterization of 16 microsatellite loci in the humbug damselfish, Dascyllus aruanus (family Pomacentridae) |
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| Authors: | Fauvelot C Smith-Keune C Jerry D R Buston P M Planes S |
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| Institution: | 1. IRD‐UR128, Centre de Biologie et d'Ecologie Tropicale et Méditerranéenne, Université de Perpignan, 52 Avenue Paul Alduy, F‐66860 Perpignan cedex, France;2. Aquaculture Genetics Research Program, School of Marine & Tropical Biology, James Cook University, Townsville, Qld 4810, Australia;3. The Integrative Ecology Group, Estación Biológica de Do?ana, CSIC, Avenida de Maria Luisa s/n Pabellón del Perú, 41013 Sevilla, Spain;4. UMR 5244 EPHE/CNRS/UPVD, Université de Perpignan, F‐66860 Perpignan cedex, France;5. UMS 2978 EPHE/CNRS, Centre de Recherches Insulaires et Observatoire de l’Environnement, BP 1013, Papetoai, Moorea, French Polynesia |
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| Abstract: | Here we report on 16 microsatellite loci designed for the damselfish Dascyllus aruanus. All loci were tested on 98 individuals and were polymorphic (seven to 35 alleles). Expected heterozygosity ranged from 0.705 to 0.942. Six loci showed Hardy-Weinberg disequilibrium due to the occurrence of null alleles. Cross-species amplifications conducted within the genus Dascyllus (D. carneus, D. strasburgi, D. trimaculatus) lead to polymorphic fragments in 32 out of 48 tests. These 16 loci will enable future research into the behavioural ecology and population ecology of Dascyllus aruanus throughout the Indo-Pacific. |
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| Keywords: | coral reef fish Dascyllus aruanus microsatellites parentage analyses Pomacentridae population structure |
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