Development and characterization of novel microsatellite markers from the Chinese rufous horseshoe bat (Rhinolophus sinicus) with cross-species amplification in closely related taxa |
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| Authors: | Liu Wenchao Zhang Jinshuo Hua Panyu Zhang Shuyi Rossiter Stephen J |
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| Institution: | School of Life Science, East China Normal University, Shanghai 200062, China, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China, School of Biological and Chemical Sciences, Queen Mary, University of London, London E1 4NS, UK. |
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| Abstract: | We isolated nine polymorphic microsatellite markers from the Chinese rufous horseshoe bat (Rhinolophus sinicus) using an enriched library method. We assessed genetic polymorphism at these loci in 42 individuals from a single population. We recorded high genetic diversity with four to 17 alleles per locus, and estimated expected and observed heterozygosity values ranging from 0.492 to 0.910 and from 0.462 to 0.881, respectively. No locus departed from Hardy-Weinberg equilibrium following Bonferroni correction, and no linkage disequilibrium was detected. Most loci successfully cross-amplified congeneric species. These loci will be used to characterize phylogeographical history of Rhinolophus sinicus in China. |
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| Keywords: | bat genetic structure microsatellite loci Rhinolophus sinicus |
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